All Victorians will be turned away from the Queensland border from Friday.
A surge in new cases of COVID-19 has pushed the state government to harden its stance on visitors from the southern state.
"From noon, July 10, visitors from Victoria will no longer gain access or be able to quarantine in Queensland," Premier Annastacia Palaszczuk said in a tweet on Thursday.
"They will be turned around."
No new cases of the disease have been recorded overnight.